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Online Ovulation Test Instructions


Our online ovulation test instructions provide all the information that is needed to use our tests. If used incorrectly, the tests will not be accurate, so please read the entire set of instructions before beginning to test. If you have any questions, please don't hesitate to contact us.


The ovulation tests are used to obtain quick visual, semi-quantitative results for early detection of Luteinizing Hormone (LH) in urine samples. The LH surge is used to predict a release of mature ovum within 24-48 hours, at which the patient is most likely to become pregnant.


Luteinizing Hormone (LH) plays important roles in the regulating the ovulation and ovarian functions during the menstrual cycle. Normally present in very small concentration in urine, LH rapidly increases in the mid cycle (commonly referred to as "ovulation surge"), usually 1 to 2 days prior to release of the mature ovum (ovulation). By monitoring the surge of LH in urine it is possible to determine the optimal chance for egg fertilization and planned pregnancy.


Prior to administering an ovulation test it is necessary to determine the length of your menstrual cycle. This is the number of days from the first day of menstrual bleeding to the day before the next bleeding starts again. Refer to the following chart to determine when you should start testing. The first day of bleeding is day 1. Perform testing 6 days in a row or until the LH surge (darkest test line) has been detected.

My cycle is:

Start testing on:

21 days day 5
22 days day 6
23 days day 7
24 days day 8
25 days day 9
26 days day 10
27 days day 11
28 days day 12
29 days day 13
30 days day 14
31 days day 15
32 days day 16
33 days day 17
34 days day 18
35 days day 19
36 days day 20
37 days day 21
38 days day 22
39 days day 23
40 days day 24


  1. Obtain a clean plastic or glass container to collect a urine sample.
  2. Collect urine once per day. LH is synthesized during the day so best time to test is 10am to 8pm. DO NOT USE FIRST MORNING URINE.
  3. Make sure to test daily at approximately the same time.
  4. Specimen should be allowed to settle for 5 minutes and reach room temperature before testing.


  1. Make sure the urine sample and unopened ovulation test are at room temperature prior to administering the test.
  2. Remove the test device from its protective pouch by tearing along a side notch.


The ovulation tests are available in different formats for easy and convenient testing. The format applications range from professional to home usage. All three tests are identical in functionality and performance, however some users may have personal preferences toward any of the formats.

Ovulation Test Strip Application
Hold the green plastic handle and immerse the test strip into the urine with the white absorbent arrow end pointing towards the sample. Make sure not to immerse past the maximum line marker. Take the strip out after 3 seconds and place it horizontally on a clean, dry, surface. Do not immerse for longer than 5 seconds. Allow 5 minutes to complete the test reaction.
Ovulation Test Cassette Application
Use the plastic pipette provided in the test pouch to withdraw the urine sample. Position the pipette over the round well opening and displace 4 drops (0.2mL) of urine by gently squeezing the pipette. Allow 5 minutes to complete the test reaction.
Ovulation Test Midstream Application
Remove the plastic cap to expose the absorbent window. Point the absorbent tip (with 5 small openings) directly in urine stream for at least 7-10 seconds to allow the sample into the testing device. (Another technique is to collect urine into a clean container and dip half of the absorbent pad for at least 10 seconds.) Re-cap the device and place it horizontally on a clean, flat surface. Allow 5 minutes to complete the test reaction.


Make sure to read test results at 5-minute mark. Reading too early or too late may yield incorrect interpretation of the results and may vary according to users experience.

Negative Result: No Ovulation Surge
Click a thumbnail to enlarge
Only one color band appears on the top Control (C) region. There should be no apparent band on the Test (T) region. The control line is designed to validate the test and should be crisp and clear in intensity with white membrane background.

Positive Results: Possible Ovulation
Click a thumbnail to enlarge
Distinct and consistent color bands appear on the Control (C) and Test (T) regions. Color intensity of the bands is directly proportional the concentration of LH hormone in urine. The test line during LH surge is usually equal to or darker than the intensity of the control line. The test line prior to and after the LH surge is usually weaker than the intensity of the control line. Therefore the pattern of darkening of the color of the test line up to the maximum intensity (usually 1-3 days) is a best predictor of ovulation surge, which in turn signifies the release of the egg (within 24-48 hours). The best time to conceive / intercourse is during this time window.
Click here for detailed explanation and graph.

Invalid Results
Uncertain test results should be discarded in case there is no visible control line. Repeat test with a new test device.


Unless otherwise specified by a fertility specialist, stop testing once the LH surge is detected.

Leftover unused tests may be saved for later use. Please remember that testing usually takes six to ten days so make sure you have sufficient number of tests to complete the procedure.

NOTE: Listed below are some of the reasons why a surge may not be detected.

  1. Urine is collected at the wrong time of day, such as first morning urine, which should not be used.
  2. The concentration of LH is too low to accurately detect. If you are consistently experiencing this problem please contact your fertility specialist.
  3. Testing is performed too early or too late in the menstrual cycle. Refer to table in the PREPARATION section of the instructions to determine when to start testing.
  4. Testing is stopped before the surge occurs, and should have been continued for a few more days. A 10-Day test kit is recommended for this reason.
  5. A LH surge did not occur during this menstrual cycle, or was missed when testing.


Sensitivity: 25 mIU/mL LH detection limit

Specificity: > 98% correlation was determined from cross reaction studies with known amounts of human Chrionic Gonadotropin Hormone (200 mIU hCG/mL ), Follicle Stimulating Hormone (200 mIU hFSH/mL), and Thyroid Stimulating Hormone (200 IU hTSH/mL). All tests yielded color much less intensive than that of 20 mlU/ml hLH Reference.

The ovulation test has been standardized to World Health Organization Second International Standard (IS 80/552).

Containing membrane coated with goat anti-LH colored colloidal gold - monoclonal mouse anti-LH conjugated predried in pad.

Interference Testing
The following substances were added in hLH free and hLH spiked urine samples. None of the substances at concentration tested interfered in the assay.

Acetaminophen 20 mg/dl
Acetylsaticylic Acid 20 mg/dl
Ascorbic Acid 20 mg/dl
Atropin 20 mg/dl
Caffeine 20 mg/dl
Gentesic Acid 20 mg/dl
Glucose 2 g/dl
Hemoglobin 1 mg/dl

Built in Quality Control
The procedural control is built in to the test. A color band appearing on the control region of the strip indicates proper test performance.


  1. The urine ovulation test is designed for in vitro diagnostic use only.
  2. Do not use the results of this test as an aid for contraception.
  3. A test device can only be used once. Discard the test after use.
  4. Always compare the color of the test band to that of the control band of the same device on the day the test is performed. Do not compare bands from different devices.
  5. Urine from pregnant women, women in menopause, or after receiving a hCG shot (injection) should not be used with this test, and will cause inaccurate results.
  6. If you are taking hormonal medications please consult with your fertility specialist.
  7. If irregular or unusually long cycles are experienced, or if you are experiencing consistent problems in detecting ovulation surge please contact your physician or fertility specialist.


The test devices should be kept from prolonged exposure to direct sunlight, moisture, heat, and other related conditions.

The test kit can be stored at normal room temperature (2C to 30C) in the sealed pouch up to the expiration date of 2 years, which is indicated on the package.


Urine samples and used test devices are potentially infectious so please keep them away for direct contact with objects. Proper handling, hands washing, and immediate disposal is recommended.


  1. Keep away from children and prolonged exposure to extreme storage conditions.
  2. This test is designed for single in vitro diagnostic use only.
  3. Do not use test kit beyond expiry date printed on package.
  4. Tests results may vary for different individuals.


Our customer service team will be glad to offer assistance so please feel free to contact us with your detailed inquiry.


  1. France, J.T. In Recent Advances in Obstetrics anGynaecoloqy Number 14, J. Bonner, ed., Churchill.Livingstone. New York, NY. 1982. pp 215-239.
  2. Moghissi KS, Syner FN, Evans TN: A composite picture of the menstrual cycle. Am J Obstet Gynecol 114:405, 1972.
  3. Speroff, L., Glass, R.H., Kase N.G. Clinical Gynecologic Endocrinology and infertility, 3rd ed. , Williams and Wilkins, Baltimore, MD, 1983.
  4. Bangham. D.R. Acta Endocrinol. 71,625-637 1972.
  5. Lundy LH, Lee SG, Levy W, et al: The ovulatory cycle: A histologic, thermal, steroid and gonadotropin correlation. Obstet Gynecol 44:14,1994.
  6. Hosseimian AH, Kim MH: Predetermination of ovulation timing luteinizing hormone assay.Fertility and Sterility 27 (4):369,1976.
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